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When working with charts, each one has a specific requirements defining what Chart Data to be used. For a 3D Stacked Column chart, the minimum number and rows are 1 numeric column with all-positive or all-negative values, as per the error message. You will find some more details regarding rows and columns as well as a Data example here.

For example, the DataSource could look something similar to the following, where the DataSource contains 4 Columns and 5 Rows:

protected void Page_Load(object sender, EventArgs e)
{
this.UltraChart1.DataSource = GetData();
this.UltraChart1.DataBind();
}

private DataTable GetData()
{
DataTable mydata = new DataTable();
// Define the columns and their names
mydata.Columns.Add("Series Label", typeof(string));
mydata.Columns.Add("Segment A", typeof(int));
mydata.Columns.Add("Segment B", typeof(int));
mydata.Columns.Add("Segment C", typeof(int));

// Add the rows of data
mydata.Rows.Add(new Object[] { "Stack A", 8, 11, 14 });
mydata.Rows.Add(new Object[] { "Stack B", 4, 11, 16 });
mydata.Rows.Add(new Object[] { "Stack C", 3, 11, 18 });
mydata.Rows.Add(new Object[] { "Stack D", 3, 11, 12 });
mydata.Rows.Add(new Object[] { "Stack E", 3, 11, 16 });
return mydata;
}

In case you are interested, here you will find several online samples illustrating Stacked Column Chart and Stacked 3D Bar/Column Chart